紫外分光光度法测定盐酸土霉素可溶性粉含量

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紫外分光光度法测定盐酸土霉素可溶性粉含量(7600字)
摘要：采用紫外分光光度法测定盐酸土霉素可溶性粉含量，结果表明:用0.1mol/L盐酸溶液做参照液，以353nm为测定波长，土霉素在8~40ug/ml的浓度范围内，浓度与吸收值呈良好的线性关系。相关系数r=0.99998,平均回收率（n=5）为100.02%，RSD=0.50%。本法与抗生素微生物检定法进行比较，测定结果无显著差异，但本法操作简便、结果准确，适用于半成品的含量检测。
关键词：紫外分光光度法；抗生素微生物检定法；盐酸土霉素可溶性粉；含量

Determination of Oxytetracycline Hydrochloride Soluble Power with UV Spectrophotometry
Abstract:UV spectrophotometry was useed for the assay of oxytetracycline in oxytetracycline hydrochloride soluble power. The results showed that with 0.1mol/L hydrochloric acid as references solution at 353 nm, the abs orbance of oxytetracycline was in a good linear correlati on ( r=0.99998) with concentrati on in range of 8~40 μg/mL, The average recovery was 100.02%, and the RSD was 0.50%( n = 5) .The results of UV spectrophotometry and antibiotics-microbial test were basically the same.But this method is simple,accurate and suitable for the determination of semi-manufactured goods.
Key words: UV spectrophotometry;antibiotics-microbial test;oxytetracycline hydrochloride soluble power; content