致病力减弱的稻瘟菌转化体SX2550和SX2581的筛选

致病力减弱的稻瘟菌转化体SX2550和SX2581的筛选(8700字)
摘 要：苗期接种法筛选出致病力减弱的稻瘟菌转化子。活化转化子，经过产孢培养，接种到有一叶苗龄的大麦苗上，观察发病叶片上病斑类型和数量，比对野生型P131阳性对照，判定菌株发病等级，进而筛选出发病程度明显减弱的菌株，即致病力减弱的稻瘟菌转化子。本研究筛选致病力减弱稻瘟菌突变体，将更好地把握稻瘟病菌致病的分子生物学及其与水稻寄主的互作关系，培育抗病品种降低稻瘟病菌。从而为开发能够抑制病原菌发展的新型药物提供一些新的靶位点。
关键字：稻瘟菌；番茄燕麦培养基；活化；接种；致病力

Screening of the reduced virulence of Magnaporthe grisea transformants SX2550 and SX2581
Abstract: Screening reduced virulence of Magnaporthe grisea transformants by seedling inoculation. mediated activation of ATMT transformants, after sporulation culture, inoculated with a large wheat seedling leaf seedling age, the observed incidence of lesion type and quantity of leaves, mutatis mutandis, the wild-type p131 positive control, determine the incidence bacteria levels, and then filter out the extent of significantly decreased the incidence of strains, that is, reduced virulence of Magnaporthe grise transformants. This study screening irulence of rice blast abate bacterium mutants, will grasp the molecular biology of magnaporthe grisea pathogenic and rice the host interactions, foster resistant varieties reduce magnaporthe grisea. Thus for development can inhibit pathogen development of new drugs provides some new sites points
Key words: Magnaporthe grisea ; tomato oats medium ; activation ; vaccination ; virulence
 

目  录
摘要    1
关键词    1
1.前言    1
1.1稻瘟菌的生活史    2
1.2 稻瘟病菌作为模式菌株的特点    3
1.3稻瘟菌的致病机制    3
1.4本研究的立题依据和研究意义    3
2 材料与方法    4
2.1 材料    4
2.1.1  供试菌株    4
2.1.2  供试培养基及配制方法    4
2.1.3  供试实验材料及器材    4
2.2 方法    5
2.2.1  突变体筛选    5
2.2.2  突变体再次验证    6
2.2.3  突变体保存    6
3 结果与分析    6
3.1  致病力减弱的突变体筛选    6
3.2  突变体生物学性状的观察    9
4 讨论    10
4.1 苗期接种发病情况影响因素分析    10
4.1.1提高菌种活化成活率    10
4.1.2提高产孢量    10
4.1.3提高接种技术    10
4.1.4培养、接种条件    11
参考文献    11
致    谢    13