稻曲病室内药剂筛选试验研究

稻曲病室内药剂筛选试验研究(7300字)
摘  要：稻曲病是一种水稻穗部病害，由稻曲病菌Ustilaginoidea virens(Cooke)Takahashi在水稻穗部为害产生稻曲球，目前已经上升为水稻产区的主要病害之一。
本研究主要为稻曲病药剂室内筛选试验研究，筛选出有效控制水稻稻曲病的药剂。采用室内离体平皿生长速率法测定药剂的杀菌毒力,测定供试药剂对稻曲病病菌的毒力,并采用最小二乘法求得其毒力回归方程。70%甲基硫菌灵EC50（ug/mL）是2.193。15%三唑酮EC50（ug/mL）是0.2791。70%代森锰锌EC50（ug/mL）是35.0720。50%多菌灵EC50（ug/mL）是0.4115。20%井冈霉素EC50（ug/mL）是0.0593。40%稻瘟灵EC50（ug/mL）是40.3554。12．5%烯唑醇EC50（ug/mL）是1.0678。43%戊唑醇EC50（ug/mL）是0.0123。30%丙环唑+苯醚甲环唑EC50（ug/mL）是0.0149。
通过室内药剂筛选实验发现，丙环唑+苯醚甲环唑、戊唑醇、烯唑醇对稻曲病菌的抑菌活性都很高，对稻曲病的防治有较好的效果。
关键词：稻曲病；培养基；毒力测定；菌落；菌丝；毒力回归方程

Ustilaginoidea virens(Cooke)Takahashi indoor elixir screening tests research
Abstract：rice song disease is a kind of disease, rice panicle rice Ustilaginoidea virens bent by bacteria in Cooke) Takahashi (rice panicle leucaena curveball, currently produces rice has risen as one of main disease division of rice.
This research mainly for the rice song disease elixir indoor screening tests were studied, effective control of rice paddy song disease potion. Using indoor vitro AGAR growth rate method for determining the sterilization irulent potion of 5day-age potions, determination of rice song disease germs virulence, and USES the least-square regression equation for its poisonous force. 70% methyl sulfur bacteria spirit EC50 (ug/ml) is 2.193. 15% sanzuotong EC50 (ug/ml) is 0.2791. Mancozeb 70% EC50 (ug/ml) is 35.0720. More than 50 percent bacteria spirit EC50 (ug/ml) is 0.4115. 20% interact EC50 (ug/ml jinggangmeisu) is 0.0593. 40% rice blast spirit EC50 (ug/ml) is 40.3554. 12.5% paclobutrazol EC50 (propylene alcohol 1.0678 ug/ml) is. 43 percent alcohol EC50 (ug paclobutrazol e/ml) is 0.0123. 30% propiconazole + benzene ether JiaHuan thiazole EC50 (ug/ml) is 0.0149.
Through the indoor elixir screening tests found that propiconazole + benzene ether JiaHuan thiazole, alcohol, xi paclobutrazol e paclobutrazol on rice song germs gliadin antibacterial activity is very high, the prevention of rice song disease has good effect.
Key words：Rice song disease; Medium; Virulence determination; Colonies;Hyphae;Virulence regression equations
 

目    录
摘要……………………………………………………………………………1
关键词…………………………………………………………………………1
1 前言……………………………………………………………………………2
1.1 症状 …………………………………………………………………3
1.2 病原 ……………………………………………………………………3
1.3 发病规律…………………………………………………………………4
1.4 病害控制…………………………………………………………………4
2 稻曲病药剂室内筛选试验…………………………………………………………6
2.1 材料和方法………………………………………………………………………6
2.1.1 供试药剂…………………………………………………………………6
      2.1.2 供试菌种…………………………………………………………………6
2.2 实验方法…………………………………………………………………………6
2.2.1 带毒培养基的制备………………………………………………………6
2.2.2 菌饼的移植 ………………………………………………………………6
2.2.3 菌落直径的测量及计算方法 …………………………………………… 6 
3 结果与分析 ………………………………………………………………………7
3.1 不同药剂的对四种病菌菌丝生长的影响 ………………………………7
3.2 不同药剂对稻曲病菌的毒力测定 ………………………………………8
4 讨论 ……………………………………………………………………………9
参考文献 ………………………………………………………………………10
致谢……………………………………………………………………………11