丁香酸对铜绿微囊藻的抑制生长作用(含选题审批表,任务书,开题报告,中期检查表,毕业论文5700字)
摘 要： 微囊藻(Misrocystis)是富营养化湖泊中形成蓝藻水华(Water bloom)的主要藻类。本实验室前期研究表明，小麦麸皮对铜绿微囊藻生长具有明显的抑制作用。丁香酸是小麦麸皮中主要的酚类物质之一,为了阐明丁香酸对小麦麸皮抑藻效应的贡献，本文研究了丁香酸对铜绿微囊藻生长和光合色素含量的影响。试验结果表明:5种质量浓度的丁香酸对铜绿微囊藻均有显著的抑制作用，浓度越大，抑藻效果越明显。以第6天的藻液为例：丁香酸质量浓度分别为10 mg•L-1、20mg•L-1、40 mg•L-1、80 mg•L-1、160mg•L-1对铜绿微囊藻细胞数相对于空白时分别下降了11%、12.5%、22.6%、46.2%、92.9%；叶绿素a分别下降了0%、1.2%、3.6%、28.2%、64.8%；类胡萝卜素分别下降了0.2%、2.5%、6.2%、29.1%、70.3%。
关键词：铜绿微囊藻,丁香酸、抑藻作用、湖泊
Inhibitory effects of syringic acid against the growth of Microcystis aeruginosa
Abstract：Microcystis is the main group cyanobactria water bloom in the eutrophic lakes.The previous results in our lab indicated that wheat bran lechate showed inhibitory effect on the growth of Microcystis aeruginosa. wheat bran to algae growth was significantly inhibit.Clove acid is the main wheat bran one of phenolic substances,to illustrate the wheat bran clove acid on algal inhibiting effect of contribute,This paper studies the clove acid on copper green algae growth and photosynthetic pigment content influence.The test results show that: five mass concentration of syringic acid,of algae acid was all had significant inhibitory effect, concentration, the bigger the algal inhibiting effect of the more obvious.On the sixth day of the algae liquid, for example:Clove acid mass concentration respectively of 10 mg • L-1, 20 mg • L-1, 40 mg • L-1, 80 mg • L-1, 160 mg • L-1 was on algae cell count, relative to when the blank were down 11%, 12.5%, 22.6%, 46.2% and 92.9%;Chlorophyll a were down 0%, 1.2%, 3.6%, 28.2% and 64.8%;Carotenoids were down 0.2%, 2.5%, 6.2%, 29.1%, 70.3%.
Key words：Microcystis aeruginosa, syringic acid, growth, inhibitory effect, lakes
目 录
摘 要： 1
关键词： 1
1 前言 2
2 材料与方法 2
2.1 供试藻种 2
2.2 主要仪器及设备 3
2.3 主要试剂 3
2.4 藻类培养基的配制 3
2.4.1 培养基营养液成分 3
2.4.2 配制方法 4
3 实验方法 4
3.1 实验器皿准备 4
3.2 原藻种的培养 4
3.3 抑藻实验 5
3.3.1 接种藻种 5
3.3.2 藻液取样 5
3.4 丁香酸对铜绿微囊藻的三种参数的测定 5
3.4.1 分光光度法测定叶绿素类胡萝卜素含量 5
3.4.2 血球计数板测细胞数 5
4 数据处理 5
5 实验结果分析 5
5.1 丁香酸对铜绿微囊藻生长的影响 5
5.2丁香酸对铜绿微囊藻细胞色素含量的影响 6
藻生长的现象 9
6 结论 9
参考文献 10
致 谢 12 |
