餐厅垃圾高效降解菌的筛选与研究应用

餐厅垃圾高效降解菌的筛选与研究应用(9300字)
摘  要 ：利用平板分离技术，以餐厅垃圾为降解基质从农药废水生化池活性污泥分离出1种细菌和1种真菌。根据菌种的耐碱度实验和降解力实验，表明真菌的降解能力相当强，而混合菌种降解效果最好。经分析，活性污泥菌胶团强大的吸附能力、真菌高效的分解能力以及混合菌群中各菌种的互生、共生关系起到了很好的协同作用，使降解效果大大提高。从菌落形态和显微镜观察到的菌体结构与形态情况看，初步确定该真菌属于子囊菌。
关键字：餐厅垃圾； 筛选；降解菌；

Research of Screening and Application on Predominated Degradation Bacteria to Restaurant Rubbish
Abstract: Two different kinds of strains are isolated from active sludge using plate screening techniques with  restaurant rubbish as the degrading material.one is a kind of ganism，the other is a kind of fungus．According to the experiment of alkaline bating and degradation，it is indicated that fungus has better degradation ability，and the mixed bacteria in proportion of 1：3 has the best function．The synergism is enhanced by the strong adsorption of the active sludge clump and the mutualism an d symbiosis relations of the mixed bacteria， an d these make the degradation function improved greatly．Seeing from the morphology of the colony an d the structure and the morphology of the bacteria，the fungus is identified as the Sac fungi.
Key words:；restaurant rubbish screen；degradation bacteria
 

目  录
摘  要    1
1.前言    2
1.1 什么是餐厅垃圾    4
1.2餐厅垃圾的主要成分及特点    4
1.3 国内外餐厅垃圾的产生及处理状况    4
1.4 目前餐厅垃圾处置的弊端    5
1.5餐厅垃圾的综合利用    5
1.6 结束语    6
2 材料    7
2.1试剂    7
2.2仪器    7
3 方法    7
3.2细菌的分离筛选    8
3.21分离    8
3.22筛选    8
3.3耐碱度实验    9
3.4降解力实验    9
3.5制作葡萄糖标准曲线并测定葡萄糖含量    10
3.6制作蛋白质标准曲线并测定蛋白质含量    10
4 结果与分析    11
4.1 葡萄糖的标准曲线    11
4.2牛血清蛋白的标准曲线    12
4.3微生物特征    13
4.4降解力实验    13
4.5耐碱度实验    13
4.6上清液中的葡萄糖含量    14
4.7上清液的蛋白质含量    15
5 结论    15
 参考文献    16
致谢    18